Journal: BMC urology
Article Title: CpG dinucleotide methylation of the SPDEF gene as a blood-based epigenetic biomarker for prostate cancer diagnosis.
doi: 10.1186/s12894-025-01824-5
Figure Lengend Snippet: Fig. 1 Visualization of the HaeIII restriction site at the cg11346722 locus, located on chromosome 6 (chr6:34544344–34544482). The recognition sequence GG^CC is highlighted within the SPDEF promoter region, and the cleavage site of HaeIII is indicated by an arrow. This illustration is based on data from NEBcutter V2.0 (https://nc2.neb.com/NEBcutter2), which was used to identify methylation-sensitive restriction enzyme (MSRE) recogni tion islands relevant to the design of the MSRE-PCR assay
Article Snippet: The restriction enzyme HaeIII (recognition site: 5′-GG^CC-3′; Takara, Shiga, Japan) was used to digest unmethylated DNA.
Techniques: Sequencing, Methylation